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 Field Name  Data Description
Test Name  Diazepam Level
Code  Diazepam Level.
CPT Code  80346
Last Modified  3/21/2018 2:56:00 PM
Test Name  Dizepam.
Synonyms  Diastat, Diazemuls, Dizac, Diastat Acudial, Intensol, Valium, nordiazepam
Patient Preparation  
Spec. Requirements  Blood
Tube  Red, Lavender(EDTA), or Drk Green(Li or Na heparin)
Collection Volume  4.0 mL Red, 4.0 mL Drk Green, or 2.0 mL Lavender
Storage  Refrigerate
Routine TAT  2-5 days
Days Test Performed  Monday - Friday
Performed by BHS  None
See Availability  
Reference Lab  LabCorp of America
Reference Lab Code  007989 Diazepam
Clinical Use  Diazepam is effective in the management of generalized anxiety disorder and panic disorder in appropriately selected patients. Its use prior to endoscopy or cardioversion relieves anxiety and diminishes the patient's recall of the procedure. Preoperative use relieves anxiety and tension and may provide anterograde amnesia. Additional uses include the treatment of skeletal muscle spasms due to inflammation or trauma, spasticity (eg, multiple sclerosis, cerebral palsy, paraplegia, stiff-man syndrome), seizure disorders (eg, status epilepticus, febrile seizures), and alleviation of abstinence symptoms during alcohol withdrawal. Diazepam is a muscle relaxant and antianxiety drug. Peak blood levels are achieved within an hour after oral dose. Half-life in adults is 21 to 37 hours. The major metabolite (nordiazepam) has a half-life in adults of 50 to 99 hours. It is the major metabolite also of clorazepate and prazepam. Minor active metabolites of diazepam are temazepam (3-hydroxydiazepam) and oxazepam (3-hydroxy-N-diazepam).
Reference Range  
Critical Value  
Reference Range  
Critical Value  
Component  Nordiazepam
Reference Range  
Critical Value  
Component  Diazepam + Nordiazepam
Reference Range  
Critical Value  
Testing Sample Type  Serum or Plasma
Min Lab Testing Volume  0.8 mL
Special Handling  Collection Transfer separated serum or plasma to a plastic transport tube. Oral: peak: one hour after dose; IV: peak: 15 minutes after dose.
Lab Notes  
Methodology  Liquid Chromatography/Tamdem Mass Spectrophotometry (LC/MS/MS)
Limitations  Rejection: Gel-barrier tube. The use of gel-barrier tubes is not recommended due to slow absorption of the drug by the gel. Depending on the specimen volume and storage time, the decrease in drug level due to absorption may be clinically significant.

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