| Field Name |
Data Description |
| Test Name |
Dibucaine, S/RBC Cholines |
| Code |
Dibucaine, S/RBC Cholines. |
|
CPT Code |
82482,82480,82638 |
| Last Modified |
3/23/2018 11:00:00 AM |
| Test Name |
DBC S/RBC. |
| Synonyms |
Cholinesterase Inhibition by Dibucaine, Pseudocholinesterase Inhibition, Dibucaine number with serum and RBC cholinesterase |
| Patient Preparation |
Do NOT collect within 24 hours of administration of muscle relaxant |
| Spec. Requirements |
Whole Blood |
| Tube |
Red or Gold AND Lavender(EDTA) |
| Collection Volume |
4.0 mL Red, 3.5 mL Gold and 2.0 mL Lavender |
| Storage |
Ambient or Refrigerate 14 days |
| Routine TAT |
|
| STAT TAT |
|
| Days Test Performed |
|
| Performed by BHS |
None |
| See Availability |
|
| Reference Lab |
LabCorp of America |
| Reference Lab Code |
121152 Dibucaine, S/RBC Cholines |
| Clinical Use |
Assess the presence of homozygous or heterozygous "atypical" cholinesterase variant, in patients who have low result of serum or plasma cholinesterase assay, and may be at risk of apnea when given succinylcholine muscle relaxant. The degree of serum or plasma cholinesterase inhibition produced by dibucaine is under genetic control. Sensitivity to succinyl choline is dependent upon at least four allelic genes. The total quantitative cholinesterase determination may not be reliable in detecting sensitivity to succinyl choline as variant enzymes exhibit qualitative and quantitative differences in substrate specificity. The dibucaine number indicates the percent inhibition of enzyme activity by this agent when a serum or plasma sample is tested under standard conditions (inhibition expressed as a percent). This approach to detection of serum or plasma cholinesterase variants does not entirely avoid the problem of variation in reactivity with some atypical enzymes. Prolonged apnea following hemodilutional cardiopulmonary bypass has been reported in a patient whose admission (preoperative) plasma cholinesterase level was slightly below the normal range. |
| Reference Range |
|
| Critical Value |
|
|
|
| Testing Sample Type |
Serum AND Whole blood |
| Min Lab Testing Volume |
0.5 mL serum AND 1.0 mL whole blood |
| Special Handling |
Collection:Separate serum from cells in red-top tube and place in transport tube marked "serum". Do NOT separate EDTA tube. |
| Lab Notes |
Stability:Room temperature. Whole blood (RBC): Stable at room temperature or refrigerated for 14 days and unstable frozen. Serum: Stable at room temperature, refrigerated, or frozen for 14 days; freeze/thaw cycles: stable x3. |
| Methodology |
S - Spectrophotometry |
| Limitations |
No single simple test currently exists that can detect all enzyme variants.
Causes of Rejection:Serum not separated from cells; serum AND whole blood specimens not sent; anticoagulants other than EDTA (RBC only); frozen whole blood received; hemolyzed serum |
