| Field Name |
Data Description |
| Test Name |
Euglobin Lysis |
| Code |
Euglobulin Lysis. |
|
CPT Code |
85360 |
| Last Modified |
4/23/2018 6:31:00 PM |
| Test Name |
Euglobulin |
| Synonyms |
ELT, Euglobin Lysis Time, |
| Patient Preparation |
Do not draw from an arm with a heparin lock or heparinized catheter. |
| Spec. Requirements |
Blood |
| Tube |
Blue (3.2% Buff. NaCitrate) or Clear Blue (3.2% Buff. NaCitrate) |
| Collection Volume |
2.7 mL Blue or 1.8 mL Clear Blue |
| Storage |
Frozen |
| Routine TAT |
3-7 days |
| STAT TAT |
|
| Days Test Performed |
THURSDAY |
| Performed by BHS |
None |
| See Availability |
|
| Reference Lab |
LabCorp of America |
| Reference Lab Code |
500055 Euglobulin Lysis Time |
| Clinical Use |
The euglobulin lysis time is a global non-specific screen ofthe fibrinolytic system. The euglobulin lysis time (ELT) test provides an overview ofthe fibrinolytic system function by measuring the time it takes for an invitro clot to dissolve in the absence of plasmin inhibitors.[6] The fibrinolytic system is initiated following activation of the contact factors in thecoagulation cascade. Fibrinolytic system activation leads tothe production of plasmin, a proteolytic enzyme capable of degrading fibrin and fibrinogen as well as other plasma proteins. Increased fibrinolytic activity is suggested by clot lysis that occurs in less than two hours. A shortened ELT result implies excessive fibrinolytic activity that may be primary or could be secondary to inflammation, malignancy, trauma, fracture, liver disease, or thrombolytic therapy. Clinical bleeding is a possible consequence of excessive fibrinolysis. Excessive fibrinolysis may result from increased levels of tissue plasminogen activator (TPA), increased plasmin activity, decreased levels of plasminogen activator inhibitor-1 (PAI-1) or decreased alpha 2-antiplasmin acitivity. The effect of fibrinolysis is the production of plasma fibrin(ogen) degradation products (FDP or FSP) and D-dimer fragments. A prolonged ELT result implies a defect in the fibrinolytic system such as elevated plasminogen activator inhibitor (PAI-1) levels, elevated levels of a2- antiplasmin, a plasminogen deficiency, or decreased tissue plasminogen activator (TPA) activity. The ELT may also be prolonged if the fibrinogen level exceeds 600 mg/dL. Inadequate fibrinolysis may be associated with superficial or deep vein thrombosis, pulmonary embolism, coronary thrombosis, transient ischemic attack, or stroke. |
| Reference Range |
|
| Critical Value |
|
|
Component |
Euglobulin Clot Lysis |
|
Reference Range |
> 60 minutes |
|
Critical Value |
|
 |
|
| Testing Sample Type |
Plasma, FROZEN |
| Min Lab Testing Volume |
1.0 mL |
| Special Handling |
Collection:CITRATED PLASMA SAMPLES SHOULD BE COLLECTED BY DOUBLE CENTRIFUGATION. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anti- coagulant with the blood. A discard tube is not required prior to collection of coagulation samples except when using a winged blood collection device (i.e. 'butterfly'), in which case a discard tube should be used. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue- top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentifuge for 10 minutes. Use a second plastic pipette to remove plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp 49482). FREEZE immediately and maintain frozen until tested. |
| Lab Notes |
DO NOT DRAW FROM LINE!
Testing is performed at Esoterix Coagulation Laboratory: UY#300403 |
| Methodology |
TURB - Turbidimetry |
| Limitations |
This is a semiquantitative assay. The diagnostic potential of euglobulin lysis times is limited by the extreme variation in lysis times among healthy individuals. Both hypofibrinogenemia and factor XIII deficiency may result in a shortened lysis time.In the case of hypofibrinogenemia, the shortened time is due to the decreased amount of fibrin to be lysed. In factor XIII deficiency, the clot is not stabilized by covalent cross-linking of fibers and can be readily lysed byplasmin. Traumatic venipuncture, prolonged stasis, or incorrect sample preparation may invalidate test results.
Causes for Rejection: Severe hemolysis; improper labeling; clotted specimen; specimen diluted with I.V. fluids; samples thawed in transit; improper sample type; sample out of stability |
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