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 Field Name  Data Description
Test Name  Legionella pneumophilia Ag DFA.
Code  Legionella Pneumophila Ag DFA.
CPT Code  87278
Last Modified  4/24/2018 12:31:00 PM
Test Name  LegPneuDFA
Synonyms  Leg Ag, Detect, LPNEU, Legion, Antigen, Legionella Smear (Direct Flour), Legionella Pneumophilia Antigens, FA Smear for Legionella Pneumophila
Patient Preparation  
Spec. Requirements  Lung Biopsy, Lung exudate, Pleural fluid, transtracheal aspirate, sputum, bronchial washing.
Tube  Sterile leakproof container or slides
Collection Volume  1.0 mL Lung exudate or 1 cc Lung biospy
Storage  Refrigerated
Routine TAT  
Days Test Performed  
Performed by BHS  None
See Availability  
Reference Lab  LabCorp of America
Reference Lab Code  085506 Legionella pneumophila DFA
Clinical Use  Determine the presence of Legionella pneumophila organisms in direct FA smear of specimen, providing rapid diagnosis Community-acquired and nosocomial infections caused by multiple serogroups of Legionella are increasingly recognized. Although culture is now possible on buffered charcoal yeast extract agar, the demonstration of organisms in tissue or brushings is the fastest way to make the diagnosis. It also has the advantage of applicability to specimens contaminated with other bacteria. Development of monoclonal antibodies have increased sensitivity and specificity. False-positive reaction has been reported in a case of pleuropulmonary tularemia and in cases of Campylobacter infection.
Reference Range  
Critical Value  Detection of specific soluble antigen present in the urine of patients with Legionnaires' disease
Component  Legionella Ag
Reference Range  Not Detected
Critical Value  
Testing Sample Type  Lung biopsy, Lung exudate, Pleural fluid, Transtracheal aspirate, Sputum, Bronchial washing
Min Lab Testing Volume  1 mL
Special Handling  Collection:Diuretic urine should not be submitted for this.Contamination with normal flora from skin or other body surfaces should be avoided.
Lab Notes  Causes for rejection: Swab specimen; inappropriate specimen transport device; improper labeling; specimen received after prolonged delay (usually more than 72 hours)
Methodology  DFA - Direct fluorescent antibody
Limitations  Test does not include Legionella species other than L pneumophila.

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