| Field Name |
Data Description |
| Test Name |
Legionella pneumophilia Ag DFA. |
| Code |
Legionella Pneumophila Ag DFA. |
|
CPT Code |
87278 |
| Last Modified |
4/24/2018 12:31:00 PM |
| Test Name |
LegPneuDFA |
| Synonyms |
Leg Ag, Detect, LPNEU, Legion, Antigen, Legionella Smear (Direct Flour), Legionella Pneumophilia Antigens, FA Smear for Legionella Pneumophila |
| Patient Preparation |
|
| Spec. Requirements |
Lung Biopsy, Lung exudate, Pleural fluid, transtracheal aspirate, sputum, bronchial washing.
|
| Tube |
Sterile leakproof container or slides |
| Collection Volume |
1.0 mL Lung exudate or 1 cc Lung biospy |
| Storage |
Refrigerated |
| Routine TAT |
|
| STAT TAT |
N/A |
| Days Test Performed |
|
| Performed by BHS |
None |
| See Availability |
|
| Reference Lab |
LabCorp of America |
| Reference Lab Code |
085506 Legionella pneumophila DFA |
| Clinical Use |
Determine the presence of Legionella pneumophila organisms in direct FA smear of specimen, providing rapid diagnosis Community-acquired and nosocomial infections caused by multiple serogroups of Legionella are increasingly recognized. Although culture is now possible on buffered charcoal yeast extract agar, the demonstration of organisms in tissue or brushings is the fastest way to make the diagnosis. It also has the advantage of applicability to specimens contaminated with other bacteria. Development of monoclonal antibodies have increased sensitivity and specificity. False-positive reaction has been reported in a case of pleuropulmonary tularemia and in cases of Campylobacter infection. |
| Reference Range |
|
| Critical Value |
Detection of specific soluble antigen present in the urine of patients with Legionnaires' disease |
|
Component |
Legionella Ag |
|
Reference Range |
Not Detected |
|
Critical Value |
|
 |
|
| Testing Sample Type |
Lung biopsy, Lung exudate, Pleural fluid, Transtracheal aspirate, Sputum, Bronchial washing |
| Min Lab Testing Volume |
1 mL |
| Special Handling |
Collection:Diuretic urine should not be submitted for this.Contamination with normal flora from skin or other body surfaces should be avoided.
|
| Lab Notes |
Causes for rejection:
Swab specimen; inappropriate specimen transport device; improper labeling; specimen received after prolonged delay (usually more than 72 hours)
|
| Methodology |
DFA - Direct fluorescent antibody |
| Limitations |
Test does not include Legionella species other than L pneumophila. |
