| Field Name |
Data Description |
| Test Name |
West Nile Virus RT PCR. |
| Code |
West Nile Virus RT PCR. |
|
CPT Code |
87798 |
| Last Modified |
5/1/2018 10:11:00 AM |
| Test Name |
West Nile Virus RT PCR. |
| Synonyms |
NGI, WNV RT, Ultraq, Wnv, Rtpcr, Nile, West, UltraQual RT-PCR |
| Patient Preparation |
|
| Spec. Requirements |
Blood or CSF |
| Tube |
Gold, Yellow-top (ACD), Lavender-top (EDTA) or Plasma Preparation Tube (PPT(TM)) |
| Collection Volume |
Gold 3.5 mL, Lavender-top (EDTA) 2.0 mL, Yellow-top (ACD) 8.5 mL, or Plasma Preparation Tube (PPT) 5.0 mL |
| Storage |
Freeze |
| Routine TAT |
|
| STAT TAT |
N/A |
| Days Test Performed |
|
| Performed by BHS |
None |
| See Availability |
|
| Reference Lab |
LabCorp of America |
| Reference Lab Code |
140005 NGI West Nile Virus RT PCR |
| Clinical Use |
Detect West Nile Virus (WNV) RNA. The direct detection of WNV is a valuable tool in determining whether a patient is infected with West Nile Virus. It is also useful in determining whether blood and blood products are free of detectable WNV prior to distribution. PCR is able to directly detect WNV RNA and does not rely on antibody markers of WNV infection, which are produced after RNA is detectable in newly infected individuals. |
| Reference Range |
|
| Critical Value |
|
|
|
| Testing Sample Type |
Plasma, Serum or CSF |
| Min Lab Testing Volume |
2.5 mL |
| Special Handling |
|
| Lab Notes |
Collection: Collect blood in gel-barrier tube, yellow-top (ACD) tube, lavender-top (EDTA) tube OR plasma preparation tube (PPT(TM)). Centrifuge blood at 800-1600xg at room temperature within one hour of draw to separate serum/plasmafrom cells. Transfer serum/plasma to a plastic screw-capped transport tube and freeze. Specimens drawn into PPT(TM) needto be centrifuged in a swinging bucket rotor centrifuge within one hour and frozen. Ship frozen. To avoid delays in avoid delays in turnaround time when requesting multiple tests on frozen samples, PLEASE SUBMIT SEPARATE FROZEN SPECIMENS FOR EACH TEST REQUESTED.
|
| Methodology |
PCR - Polymerase chain reaction |
| Limitations |
Mean Detection = 7.43 copies/mL 95% Detection Cutoff = 28.16 copies/mL |
|
